The RRRC genotypes all lines upon arrival. When provided, genotyping protocols supplied by donating investigators are validated and optimized. When no genotyping protocol exists, the RRRC develops new gene-specific assays using a variety of techniques including standard PCR, RFLP analysis, High-Resolution Melt (HRM), RT-PCR, qPCR and Taqman (probe-based) methods.
For lines involving unique or uncharacterized genetic backgrounds and/or lines developed for QTL analysis which do not involve discrete genetic mutations, protocols involving genome-wide microsatellite analysis are developed.
Genotyping protocols for individual rat models are available under "genetic description" in the strain profile.
RRRC genotyping protocols have been optimized for the reagents and conditions described in the protocols. To genotype animals, the RRRC recommends researchers validate the assays independently upon receipt of animals into their facility. Reaction cycling temperatures and times may require additional optimization based on the specific genotyping reagents and instrumentation used.
Please contact us if you have any questions or need assistance- our genotyping experts are alway available for consultation and can help you trouble-shoot your genotyping assays.
10.03.2025
Plan ahead for the holidays
The University of Missouri, home of the RRRC, is closed for business for the holidays at the end of December/beginning of January. Please be aware that we will not be responding to inquiries, providing services or shipping materials from December 22, 2025 through January 2, 2026.
08.25.2025
Why Research Resource Identifiers (RRIDs) Are Important
RRIDs are unique identifiers for key biological resources, genetically enigineered animals and reagents like antibodies. Piekniewska et al. document how use of RRIDs improves trackability. Importantly, better citation practices increase research rigor and reproducibility.
07.30.2025
Animal Models and New Approach Methodologies (NAMs)
NAMs are lab (e.g., organoids and iPSCs) or computer-based methods intended to expand our tools for modeling human biology and disease. While this field is rapidly evolving, the need for animal models remains, especially for situations where a complex biological system is necessary. In addition, animal models serve as vital in vivo controls for the validation and verification of emerging NAMs.
02.16.2025
In Need of a New Rat Model?
Need a new rat model? The RRRC in conjunction with the MU Animal Modeling Core (AMC) can assist with the generation of transgenic rats, create knock-out and knock-in rats using CRISPR/Cas9 technology and genetically manipulate rat embryonic stem cells to make chimeric animals. Contact us to discuss your rat model needs and how we can help.