The RRRC genotypes all lines upon arrival.  When provided, genotyping protocols supplied by donating investigators are validated and optimized.  When no genotyping protocol exists, the RRRC develops new gene-specific assays using a variety of techniques including standard PCR, RFLP analysis, High-Resolution Melt (HRM), RT-PCR, qPCR and Taqman (probe-based) methods.

For lines involving unique or uncharacterized genetic backgrounds and/or lines developed for QTL analysis which do not involve discrete genetic mutations, protocols involving genome-wide microsatellite analysis are developed.  

Genotyping protocols for individual rat models are available under "genetic description" in the strain profile.

RRRC genotyping protocols have been optimized for the reagents and conditions described in the protocols. To genotype animals, the RRRC recommends researchers validate the assays independently upon receipt of animals into their facility. Reaction cycling temperatures and times may require additional optimization based on the specific genotyping reagents and instrumentation used.

Please contact us if you have any questions or need assistance- our genotyping experts are alway available for consultation and can help you trouble-shoot your genotyping assays.